Graziane Nicholas, Dong Yan (eds.) Electrophysiological Analysis of Synaptic Transmission

2nd edition. — Humana Press, 2022. — 283 p. — (Neuromethods 187). — ISBN 978-1-0716-2588-0.This second edition volume expands on the previous edition with discussions on the latest techniques used to study synaptic transmissions. The chapters in this book are organized into six parts. Part One looks at the basic concepts, such as extracellular and intracellular recordings, and spatiotemporal effects of synaptic currents. Part Two describes the recording of synaptic currents, such as measuring kinetics of synaptic current and measuring reversal potentials. Part Three discusses basic experimentations of synaptic transmission and covers run-up and run-down, and amplitude. Parts Four and Five cover experimentations with computational components and molecular and visual components, such as measurement of a single synapse and electrophysiological and visual tags. Part Six explores in vivo recordings and talks about general considerations for in vivo exploration of synaptic plasticity. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and thorough, Electrophysiological Analysis of Synaptic Transmission, Second Edition is a valuable resource that introduces graduate students and postdoctoral fellows to important topics in this field and also expands these topics to practical electrophysiological approaches.Basic Concepts
Extracellular and Intracellular Recordings
Electrical Theory
Amplifiers
Salt Environment
Patch Pipettes (Micropipettes)
Spatiotemporal Effects of Synaptic Current
Perforated Patch
Recording of Synaptic Current
Isolation of Synaptic Current
Fast and Slow Synaptic Currents
Measuring Kinetics of Synaptic Current
Measuring Presynaptic Release Probability
Long-Term Measurements
Measuring Reversal Potentials
Basic Experimentations of Synaptic Transmission
Amplitude
Pre-vs.​ Post-synaptic Effect
Run-Up and Run-Down
Kinetics of Synaptic Current
Experimentations with Computational Components
Measurement of a Single Synapse
Measurement of Silent Synapses
Dendritic Patch
Experimentations with Molecular and Visual Components
Electrophysiolog​ical and Visual Tags
In Vivo Recordings
Extracellular Recordings
General Considerations for In Vivo Exploration of Synaptic Plasticity
Technical Considerations for In Vivo Electrophysiolog​y